Two-Dimensional Electrophoresis with Carrier Ampholytes
نویسندگان
چکیده
منابع مشابه
Effects of ampholyte concentration on protein behavior in on-chip isoelectric focusing.
The effects of mobility corrections on carrier ampholytes are studied at various ampholyte concentrations to understand protein behavior during IEF. IEF simulations are conducted in the presence of 25 biprotic carrier ampholytes within a pH range of 6-9 after applying the Onsager-Debye-Hückel correction to the carrier ampholytes. Two model proteins with ten charge states but without ionic stren...
متن کاملTwo-dimensional electrophoresis with immobilized pH gradients: current state.
Two-dimensional (2-D) electrophoresis as it was introduced by O’Farrell in 1975 [ 11 is unsurpassed by any other technique for simultaneously resolving hundreds of polypeptides. The usefulness of this method has been documented in thousands of publications. However, 2-D electrophoresis is still a demanding procedure with respect to performance and required expertise [2-41. Because of the large ...
متن کاملSolubilization of proteins from human lymph node tissue and two-dimensional gel storage.
In the present study, we compared six different solubilization buffers and optimized two-dimensional electrophoresis (2-DE) conditions for human lymph node proteins. In addition, we developed a simple protocol for 2-D gel storage. Efficient solubilization was obtained with lysis buffers containing (a) 8 M urea, 4% CHAPS (3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate), 40 mM Tris ba...
متن کاملCapillary isoelectric focusing-mass spectrometry for shotgun approach in proteomics.
We report on capillary isoelectric focusing-mass spectrometry (CIEF-MS) of complex peptide mixtures in the absence of carrier ampholytes. Furthermore, the use of low concentrations of carrier ampholytes as mere spacers is investigated. Carrier ampholytes are complex mixtures of amphoteric compounds with high buffering capacity. Since all peptides are amphoteric compounds by themselves, the use ...
متن کاملCapillary electrophoresis as a second dimension to isoelectric focusing for peptide separation.
Capillary zone electrophoresis and carrier ampholytes based capillary electrophoresis have been used as a second separation step to Off-Gel isoelectric focusing for the analysis of complex peptide mixtures. A tryptic digest of four proteins (bovine serum albumin, beta-lactoglobulin, horse myoglobin, cytochrome c) has been chosen as a peptide test mixture. After assessment of different modes of ...
متن کامل